EFFECTS OF ULTRASOUND EXPOSURE IN VITRO ON NEUROBLASTOMA CELL MEMBRANES

作者: Margaret Fahnestock , Virginia G. Rimer , Ruth M. Yamawaki , Pepi Ross , Peter D. Edmonds

DOI: 10.1016/0301-5629(89)90162-2

关键词:

摘要: Mechanisms of action ultrasound on cell membranes were studied two murine C1300 neuroblastoma lines minimum genetic diversity. Cavitation was established in rotating polystyrene centrifuge tubes by 1 MHz cw ultrasound; exposure time 5 minutes. NS20Y and N2A cells exposed suspension responded similarly 86Rb+ transport Na+-K+-ATPase activity assays, but differently trypan blue dye exclusion lysis assays. This indicates similar overall damage to the membranes, despite use trypsin release N2As only. Primary evidence NS20Ys permeabilization N2As. These results indicate that same conditions can produce different effects differ their membrane properties.

参考文章(23)
Charles C. Church, H.G. Flynn, M.W. Miller, P.G. Sacks, The exposure vessel as a factor in ultrasonically-induced mammalian cell lysis—II. An explanation of the need to rotate exposure tubes Ultrasound in Medicine & Biology. ,vol. 8, pp. 299- 309 ,(1982) , 10.1016/0301-5629(82)90036-9
S. Pinamonti, P.E. Gallenga, V. Mazzeo, Effect of pulsed ultrasound on human erythrocytes in vitro. Ultrasound in Medicine and Biology. ,vol. 8, pp. 631- 638 ,(1982) , 10.1016/0301-5629(82)90120-X
Barry Mills, Joseph T. Tupper, Cation permeability and ouabain-insensitive cation flux in the Ehrlich ascites tumor cell. The Journal of Membrane Biology. ,vol. 20, pp. 75- 97 ,(1975) , 10.1007/BF01870629
E. A. Neppiras, Subharmonic and Other Low‐Frequency Emission from Bubbles in Sound‐Irradiated Liquids The Journal of the Acoustical Society of America. ,vol. 46, pp. 587- 601 ,(1969) , 10.1121/1.1911735
P.D. Edmonds, Pepi Ross, Acoustic emission as a measure of exposure of suspended cells in vitro Ultrasound in Medicine and Biology. ,vol. 12, pp. 297- 305 ,(1986) , 10.1016/0301-5629(86)90339-X
D.F. Webster, J.B. Pond, Mary Dyson, W. Harvey, The role of cavitation in the in vitro stimulation of protein synthesis in human fibroblasts by ultrasound. Ultrasound in Medicine and Biology. ,vol. 4, pp. 343- 351 ,(1978) , 10.1016/0301-5629(78)90023-6
John Paul, Cell and tissue culture Cell and tissue culture. ,(1970)
Clive Palfrey, U.Z. Littauer, Sodium-dependent efflux of K+ and Rb+ through the activated sodium channel of neuroblastoma cells Biochemical and Biophysical Research Communications. ,vol. 72, pp. 209- 215 ,(1976) , 10.1016/0006-291X(76)90981-5
W.H. Moolenaar, C.L. Mummery, P.T. van der Saag, S.W. de Laat, Rapid ionic events and the initiation of growth in serum-stimulated neuroblastoma cells Cell. ,vol. 23, pp. 789- 798 ,(1981) , 10.1016/0092-8674(81)90443-8
Anthony Eller, H. G. Flynn, Generation of Subharmonics of Order One‐Half by Bubbles in a Sound Field The Journal of the Acoustical Society of America. ,vol. 46, pp. 722- 727 ,(1969) , 10.1121/1.1911753