Two-dimensional DNA electrophoresis: state of the art and applications

摘要: Abstract Two-dimensional DNA electrophoresis allows the analysis of multiple fragments based on both their size and sequence properties. The often complex gel separation patterns represent information-rich data sets that may be analyzed with dedicated image systems. introduction a denaturing gradient in second dimension enhances capability method to identify variations, recent construction automated instruments opens up new ways for diffusion procedure into laboratory routine. In this review, state art technique, its general principles basic methodology will discussed together major applications different fields, including environmental microbiology, mutation detection human genetics.