HORMONAL CONTROL OF LYSOSOMAL ENZYME RELEASE FROM HUMAN NEUTROPHILS : EFFECTS OF AUTONOMIC AGENTS ON ENZYME RELEASE, PHAGOCYTOSIS, AND CYCLIC NUCLEOTIDE LEVELS

摘要: The purpose of this investigation was to examine the effects autonomic neurohormones, cyclic nucleotides, and related agents on immunologic discharge lysosomal enzymes from, phagocytosis by, purified human neutrophils. In order discern possible intracellular mechanisms by which certain neurohormones influence neutrophil function, concentrations AMP GMP in neutrophils were assessed during cell contact with phagocytizable particles agents. model system employed for study interaction rheumatoid arthritic (RA) serum-treated zymosan at 37°C a neutral, balanced salt solution containing glucose. Neutrophils ingested discharged s-glucuronidase but not lactate dehydrogenase activity 30 min incubation. Treatment RA serum more effective than treatment normal regard extent both particle uptake enzyme release. During epinephrine, isoproterenol, inhibited ingestion discharge. These actions epinephrine associated concomitant elevation levels. contrast catecholamines AMP, acetylcholine accelerated release without affecting uptake. Increases levels also provoked absence added cholinergic data suggest that from can be regulated perhaps via selective formation appropriate nucleotides.