Clonal stability of Pasteurella multocida in free-range layers affected by fowl cholera.

摘要: Detailed longitudinal studies of the genetic stability Pasteurella multocida ssp. multocida, cause fowl cholera, have not previously been carried out. Consequently, aim present study was to provide detailed information on and diversity P. in poultry flocks over time, enabling new insights into molecular epidemiology this important pathogen. Longitudinal investigations rate causes mortality were out two free-range layer farms (A B) a period 11 months. The total flocks, A1 A2, farm A 62 91%, respectively, while single flock B1 B 6%. Postmortem examinations performed 708 layers from A2 159 B1. Fowl cholera main both farms. isolates recovered characterized phenotypically genotypically, 322 identified as multocida. 99 31 by restriction endonuclease analysis amplified fragment length polymorphism analysis. each had unique type, suggesting introduction successful clone. Furthermore clone identical clones isolated outbreaks avifauna Denmark 1996 2001 Sweden 1998. This provides convincing evidence for clonal outbreak