Use of Trichomonas vaginalis Clinical Isolates to Evaluate Correlation of Gene Expression and Metronidazole Resistance

摘要: We investigated whether variations in gene expression of enzymes associated with anaerobic resistance laboratory-derived strains Trichomonas vaginalis could be detected a group 28 clinical isolates metronidazole sensitivity. compared by real-time PCR because this method allows for highly sensitive quantification mRNA and evaluation several genes simultaneously. found that PFOR A levels were correlated B levels, as well the D subunit malic enzyme ferrodoxin. Ferrodoxin was also significantly hydrogenase. However, when we evaluated relationships between these to metronidazole, no significant correlations aerobic or vitro sensitivity drug any tested. Similarly, using Student's t-test, differences observed separated susceptibility. The lack correlation susceptibility result at protein level other biochemical pathways are involved settings.