Centrifugal method and apparatus for isolating a substance from a mixture of substances in a sample liquid

作者: Maxim V. Myakishev , George I. Kapanadze , George P. Georgiev , David R. Beritashvili , Gennady M. Ershov

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摘要: A centrifugal rotor effects the isolation, in a sequence of steps, substance from mixture substances dissolved, suspended or dispersed sample liquid. Multiple samples are processed simultaneously by means plurality fractionation cells, each which contains series interconnected, chambered and vented compartments individual steps isolation procedure take place. Specific preferred embodiments include lysis, sedimentation, aggregation, sorption, rinsing, desorption. The specific compartment occupied liquid one its fractions at any stage process is governed speed direction rotation gravitational force. interconnections, chambers passages sized angled to prevent predetermined amounts reagent liquids overflowing compartment. Predetermined required contact delivered every cell dynamic flow distributor accepts partially aerosolized injections said reagents pipetted dosator while rotating. method using isolate deoxyribonucleic acid described. operation centrifuge can be controlled coordinated automatically.

参考文章(26)
Josef Blum, Albert Szent-Gyorgyi, Centrifuging apparatus and system ,(1956)
Sigmar Klose, Karlheinz Mann, Hermann Edelmann, Anton Pasch, Hans-Peter Haar, Centrifugal analyzer rotor unit and insert elements ,(1981)
Harvey J. Palmer, Richard L. Columbus, Blood collection and centrifugal separation device including a valve ,(1990)
Steven Robertson, Dynamic braking centrifuge ,(1988)
Deborah Paula Freyler, Susan Melissa Atwood, Harvey John Palmer, Richard Lewis Columbus, Blood collection method ,(1990)