Reconstitution of a formate-NADP+ oxidoreductase from formate dehydrogenase and a 5-deazaflavin-linked NADP+ reductase isolated from Methanococcus vannielii.

摘要: Abstract The formate-dependent reduction of NADP+ by extracts Methanococcus vannielii is catalyzed a coupled system consisting formate dehydrogenase, 5-deazaflavin cofactor, and 5-deazaflavin-dependent reductase. All three components were purified from crude M. vannielii. Recombination these reconstituted the formate-NADP+ oxidoreductase system. dehydrogenase also can utilize FAD, FMN, number artificial dyes as electron acceptors, but do not replace cofactor in enzyme reduced form binds readily to reductase apoprotein dissociated ammonium sulfate treatment at neutral pH under anaerobic conditions. This transfer identical many its properties isolated other methane-producing bacteria.