A review on preparative and semi-preparative offgel electrophoresis for multidimensional protein/peptide assessment

摘要: Mass spectrometry (MS) techniques are commonly used for protein identification and further analysis of selected spots after high resolution 2-D electrophoresis. Complementary gel-free approaches have been developed during the last few years shown to be useful tools in modern proteomics. The development application various electrophoresis devices performing fractionation according pI differences is therefore a topic interest. This review describes current state isoelectric focusing (IEF) based on Agilent offgel 3100 fractionator. includes, therefore, (i) an overview IEF as well other previous developments; (ii) fundamentals future trends; (iii) advantages disadvantages procedures; (iv) requirements isolated pellets fractionation; (v) fraction perform second dimensional by advance chromatographic techniques; (vi) effect operating conditions stability metal-protein complexes.