Molecular Characterization of the Burkholderia cenocepacia dcw Operon and FtsZ Interactors as New Targets for Novel Antimicrobial Design

摘要: The worldwide spread of antimicrobial resistance highlights the need new druggable cellular targets. increasing knowledge bacterial cell division suggested potentiality this pathway as a pool alternative drug targets, mainly based on essentiality these proteins, well divergence from their eukaryotic counterparts. People suffering cystic fibrosis are particularly challenged by lack antibiotic alternatives. Among opportunistic pathogens that colonize lungs patients, Burkholderia cenocepacia is well-known multi-drug resistant bacterium, difficult to treat. Here we describe organization its wall (dcw) cluster: found 15 genes dcw operon can be transcribed polycistronic mRNA mraZ ftsZ and transcription under control strong promoter regulated MraZ. B. J2315 FtsZ was also shown interact with other components divisome machinery, few differences respect bacteria, such direct interaction FtsQ. Using an in vitro sedimentation assay, validated role SulA inhibitor, roles FtsA ZipA tethers polymers. Together our results pave way for future design