Proteomic analysis of the effects of gibberellin on increased fruit sink strength in Asian pear (Pyrus pyrifolia)

摘要: Abstract Gibberellin (GA) is commonly used to improve crop yield and quality in tree fruit production. To better understand the mechanism by which GA increases size at protein level, fruits from 11-year-old Asian pear cv. ‘Cuiguan’ (Pyrus pyrifolia Nakai) plants were treated with (30 mg L−1) on 30 days after anthesis (DAA). Fruits sampled early middle stages of expansion maturation stage for proteomic analysis. Our results revealed that 115 spots showed significant differences abundance between GA-treated untreated fruits. Among these spots, 60 proteins successfully identified matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI–TOF MS). The up-regulated observed related primary metabolism, transport, ion channels stress responses. During process, down-regulated photorespiration, epigenetics glycolysis. greatest changes occurred expansion. Seventeen highly abundant accumulated application sink strength further validated quantitative real time PCR. Increased levels H2O2, associated advanced cell wall loosening maturation, application. Microscopic observations mesocarp supported finding has promoting effects Increases activities ATPase, Rubisco, serine hydroxymethyltransferases NAD-dependent formate dehydrogenase significantly correlated sugar accumulation. In addition, increased vacuole-associated annexin, proteasome adenylate kinase closely involved process. subsequent validation describes a dynamic network supporting hypothesis during rapid growth both activity, resulting larger P. pyrifolia.