Modulation of cisplatin resistance in human malignant melanoma cells.

摘要: Previous studies have shown that the combination of dacarbazine, carmustine, cisplatin (DDP), and tamoxifen (TAM) produced a 53% overall response rate in patients with disseminated melanoma. Deletion TAM from regimen resulted fall to 10%, reincorporation returned 52%, suggesting an important role for TAM. Using human melanoma cell line T-289, we examined nature interaction between each member this clonogenic assays soft agar. The DDP was highly synergistic as demonstrated by median effect analysis, whereas antagonistic carmustine activated form dacarbazine. mean index at 50% kill 0.26 +/- 0.02 (mean SD) DDP. This marked synergism observed concentrations are clinically achievable. had no on uptake analogue [3H]dichloro(ethylenediamine)platinum(II). There formation or repair intrastrand DNA adducts. Similarly, there intracellular glutathione metallothioneins. We conclude is truly accomplished through none four mechanisms commonly associated resistance.