Effect of Phorbol Esters on Cytosolic Ca2+ Level, Myosin Phosphorylation and Muscle Tension in High K+-Stimulated Bovine Tracheal Smooth Muscle.
作者： Masaomi Tajimi , Masatoshi Hori , Hiroshi Ozaki , Hideaki Karaki
DOI: 10.1254/JJP.74.195
关键词:
摘要: To determine the role of protein kinase C (PKC) in bovine tracheal smooth muscle contractility, we examined effects phorbol esters on cytosolic Ca2+ level ([Ca2+]i), myosin light chain (MLC) phosphorylation and contractile force intact contraction a permeabilized preparation. In muscle, 12-deoxyphorbol 13-isobutyrate (DPB, 1 microM) increased without changing [Ca2+]i. High K+ (72.7 mM) induced sustained with increase stimulated by high K+, 50 nM DPB [Ca2+]i, microM decreasing Thus shifted [Ca2+]i/force relationship for to lower [Ca2+]i concentration-dependent manner. did not induce absence (< < 0 nM), but Ca2+/force levels. (50 MLC phosphorylation/force relationship. (1 PKC activity estimated translocation from cytoplasm membrane. These results suggest that increases sensitivity via activation PKC. Furthermore, at higher concentration has an inhibitory effect
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