Impedimetric gene assay for BCR/ABL transcripts in plasmids of patients with chronic myeloid leukemia

摘要: A label-free impedimetric biosensor was developed for determination of BCR/ABL transcripts. Specific DNA primers were covalently immobilized on a gold electrode modified with carboxylated multiwalled carbon nanotubes (cMWCNTs) and zinc oxide nanoparticles (ZnO-NPs). Aggregation the ZnO-NPs is prevented by introduction an amino-modified silica coating, which also allows subsequent covalent linkage to cMWCNTs. The typically operated at working voltage +10 mV vs. Ag/AgCl, in frequency range from 100 mHz 100 kHz. Studies surface morphology electrochemical properties demonstrated improved bioactivity. Amperometric currents parameters, such as charge transfer resistance, varied significantly throughout construction biosensor. hybridization process evidenced changes topography after exposure samples containing BCR/ABL. gene sensor has linear concentration target 6.94 aM 694 fM limit detection low 0.039 aM. Also, selective reproducible standard deviation 4.1%. Three replicates each experimental condition used. Hence, it perceived be viable tool early-stage diagnosis fusion monitoring major molecular remission clinical samples.