THE EXTRAGENOMIC ACTION OF PROGESTERONE ON HUMAN SPERMATOZOA : EVIDENCE FOR A UBIQUITOUS RESPONSE THAT IS RAPIDLY DOWN-REGULATED

摘要: Image analysis techniques have been used to demonstrate that progesterone induces a rapid calcium transient in the acrosomal domain of greater than 90% human spermatozoa (n = 2354). These results are at variance with previous reports, suggesting receptors only expressed on small subpopulation these cells, by virtue their ability bind fluorescent probes incorporating 3- (O-carboxymethyl) oxime conjugated BSA. In present study, we could confirm such bound proportion (3.01 +/- 0.29%; n 7557) although 91.79 1.8% same sperm populations exhibited response progesterone. indicate binding labeled conjugates does not reflect size responsive population; elicited this steroid is essentially ubiquitous. Progesterone action was shown involve an influx extracellular ...