Association of haplotype combination of serotonin transporter gene polymorphisms with monthly headache days in MOH patients

摘要: Objectives:  To evaluate the role of 5-HTTLPR, STin2 VNTR, and rs1042173T>G polymorphisms serotonin transporter gene (SLC6A4) as susceptibility factors for medication overuse headache (MOH) to assess their value predictors number days per month, a potential marker disease severity. Methods:  Genotyping was performed by PCR PCR-RFLP on genomic DNA extracted from peripheral blood 227 MOH patients 312 control subjects. Logistic regression analysis used association between SL6A4 risk. The polymorphic variants monthly evaluated linear analysis. Results:  analysis, adjusted age gender, revealed nominal risk (TT vs. TG + GG, OR: 1.58 95% CI: 1.05–2.37, P = 0.028). In age, primary diagnosis, acute drug overused number, VNTR found nominally associated with (12/12 others, difference: 1.55 days, 0.01–3.08, P = 0.050). When were analyzed in haplotypic combination, global haplotype emerged which remained significant after Bonferroni correction multiple comparisons (global P = 0.0056). Conclusion:  Although minor contribution SLC6A4 genetic liability cannot be excluded, haplotype-based allowed identify subgroup higher and, possibly, more severe disease.