Cell culture support containing a cell adhesion factor and a positively-charged molecule
作者： Wei-Shou Hu , David L. Clapper
DOI:
关键词:
摘要: A combination of a cell adhesion factor and positively-charged molecule are bound to the surface culture support bioreactor improve attachment stabilize growth. The positively charged is preferably polylysine, chitosan, poly(ethyleneimine) or acrylics polymerized from acrylamide methacrylamide incorporating groups in form primary, secondary tertiary amines, quaternary salts. fibronectin, laminin, collagen, vitronectin tenascin, fragments analogs having binding domain thereof. can be covalently supporting surface. In another embodiment, one either Also, both cam provided stable coating non-covalently around support. may microcarrier made polystyrene polypropylene
lens.org参考文章(15)
M. Butler, Growth limitations in microcarrier cultures. Advances in Biochemical Engineering \/ Biotechnology. ,vol. 34, pp. 57- 84 ,(1987) , 10.1007/BFB0000673
Ching-San Lai, Else G. Ankel, Larry E. Hopwood, Membrane fluidity of Chinese hamster ovary cells on plasma fibronectin-coated microcarriers Experimental Cell Research. ,vol. 150, pp. 77- 83 ,(1984) , 10.1016/0014-4827(84)90703-1
Stephane Goetghebeur, Wei-Shou Hu, Method for aggregating cells with small microspheres ,(1990)
William G. Thilly, Jason S. Wong, Daniel I. C. Wang, David W. Levine, Cell culture microcarriers ,(1979)
William G. Thilly, Daniel I. C. Wang, David W. Levine, Treatment of cell culture microcarries ,(1976)
Frederick Grinnell, Cellular adhesiveness and extracellular substrata. International Review of Cytology-a Survey of Cell Biology. ,vol. 53, pp. 65- 144 ,(1978) , 10.1016/S0074-7696(08)62241-X
Jeannette Graf, Yukihide Iwamoto, Makoto Sasaki, George R. Martin, Hynda K. Kleinman, Frank A. Robey, Yoshihiko Yamada, Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding Cell. ,vol. 48, pp. 989- 996 ,(1987) , 10.1016/0092-8674(87)90707-0
Erkki Ruoslahti, Michael D. Pierschbacher, Arg-Gly-Asp: a versatile cell recognition signal. Cell. ,vol. 44, pp. 517- 518 ,(1986) , 10.1016/0092-8674(86)90259-X
H.K. Kleinman, L. Luckenbill-Edds, F.W. Cannon, G.C. Sephel, Use of extracellular matrix components for cell culture Analytical Biochemistry. ,vol. 166, pp. 1- 13 ,(1987) , 10.1016/0003-2697(87)90538-0
A. L. VAN WEZEL, Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature. ,vol. 216, pp. 64- 65 ,(1967) , 10.1038/216064A0