Drosophila C Virus Systemic Infection Leads to Intestinal Obstruction

摘要: Drosophila C virus (DCV) is a positive-sense RNA belonging to the Dicistroviridae family. This natural pathogen of model organism melanogaster commonly used investigate antiviral host defense in flies, which involves both interference and inducible responses. Although lethality routinely as readout for efficiency immune response these studies, virus-induced pathologies flies still are poorly understood. Here, we characterize pathogenesis associated with systemic DCV infection. Comparison transcriptome infected or two other viruses, Flock House Sindbis virus, reveals that infection, unlike those represses expression large number genes. Several genes expressed specifically midgut also repressed by starvation. We show infection triggers nutritional stress results from intestinal obstruction accumulation peritrophic matrix at entry food ingested crop, blind muscular storage organ. The related cricket paralysis (CrPV), efficiently grows Drosophila, does not trigger this pathology. DCV, but CrPV, infects smooth muscles surrounding causing extensive cytopathology strongly reducing rate contractions. conclude tropism an important organ within foregut dipteran insects, crop. IMPORTANCE one few identified viral pathogens affecting melanogaster. As such, it deciphering host-virus interactions insects. here essential characterized digestive tract, crop. Our may have relevance members Dicistroviridae, some pathogenic beneficial pest insect species.