Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers.

摘要: Abstract Coke oven workers are exposed to high levels of carcinogenic polycyclic aromatic hydrocarbons, including benzo[a]pyrene (B[a]P), and at increased risk lung cancer. Since B[a]P is enzymatically activated 7 beta,8 alpha-dihydroxy(9 alpha, 10 alpha)epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]PDE) that forms adducts with DNA, the presence these was measured in DNA from peripheral blood lymphocytes by synchronous fluorescence spectrophotometry enzyme radioimmunoassay. Approximately two-thirds had detectable B[a]PDE-DNA adducts. Antibodies were also found serum 27% workers. not antibodies detected sera a control group nonsmoking laboratory and/or indicate exposure its metabolic activation metabolite covalently binds damages DNA. Detection them may be useful as internal dosimeters pathobiological effective doses chemical carcinogens.