Separation of neutral glycosphingolipids and sulfatides by thin-layer chromatography.

摘要: Two one-dimensional systems for separation of glycolipids from total lipid extracts tissues by thin-layer chromatography are described. System I used, as adsorbent, an alkaline mixture silica gel without CaSO(4) binder (75%) and magnesium silicate (25%), the lipids were "developed" with three successive solvent mixtures. The separated compounds (from fastest to slowest moving) were: ceramide, ceramide monohexosides, sulfatides, dihexosides, psychosine, trihexosides, N-acetylhexosamine trihexosides. In system II a two-step development was used on adsorbent consisting (80%) (20%). ceramides, dihexosides. Psychosine sulfatides well trihexosides not separated. both all neutral moved very top chromatogram phospholipids stayed at origin. Application demonstrated animal tissues.