Characterization of AtST4c function in flowering in Arabidopsis thaliana

摘要: A main interest of our laboratory is to characterize the function 17 sulfotransferase-coding genes Arabidopsis thaliana. The purpose my project elucidate biochemical and biological functions AtST4c, a member AtST4 subfamily (AtST4a, b c). AtST4c knockout plants were found exhibit photoperiod-independent early flowering phenotype suggesting that plays negative role in induction. In addition, produced shorter primary roots, reduced number lateral slightly smaller rosettes, fewer seeds per silique finally seeds, positive growth. Transcript expression studies showed mainly expressed roots repressed by cytokinin trans-zeatin effect on plant growth signaling pathway Arabidopsis. In order further control time, floral integrator such as LEAFY, SUPPRESSOR OF OVEREXPRESSION CO 1(SOC1) APETALA 1 was studied mutant plants. Our results show up-regulation LEAFY acts upstream these two important meristem identity genes. However, no changes observed independent four five pathways promote Taken together suggest participates time via aging or interfering with repression mediated gene TERMINAL FLOWER 1. To we compared sulfated meatbolome wild-type using liquid chromatography-mass spectrometry. Using this approach able propose structure for substrate AtST4c.