Methodological Challenges of Protein Analysis in Blood Serum and Cerebrospinal Fluid by Capillary Electrophoresis

摘要: High-performance capillary electrophoresis (HPCE or CE) is an ultrasensitive analytical technique with high resolving power and a wide area of applications including peptide/protein analysis. Its applicability greatly enhanced by the short separation times, ease method development minimum sample organic solvent requirements. Various HPCE modes have been developed for analysis, zone electrophoresis, micellar elektrokinetic chromatography, isoelectric focusing, isotachophoresis, gel microemulsion chromatography. can easily be applied to quality control manufacturing processes clinical routine diagnostic purposes due its potential provide information on identity, purity samples quantities constituents. Furthermore, interactions peptide protein other molecules studied HPCE. The principles various operation analysis are presented in this article. order exemplify application serum which importance practice, electrophoretic methods cerebrospinal fluid proteins also reviewed.