BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?

摘要: Immunoglobulin (Ig) E is the critical effector molecule in allergic reactions. Consequently, research efforts to understand biology of IgE-expressing cells paramount importance. In particular, role IgE+ memory B (MBCs) perpetuation reactivity has been subject intense research. Studies mice have convincingly established that are rare and transient and, therefore, an unlikely candidate maintain disease. contrast, MBCs detected by flow cytometry sputum peripheral blood humans proposed as a clinical marker We method genetically validate, at single-cell level, putative identified from humans. We, then used this information develop enhanced protocol more accurately identifies bona fide MBCs. found were some patients with atopic dermatitis, but frequency was ~100 times lower than previously reported. also undetectable PBMCs peanut patients. These findings provide tools identify MBCs, demonstrate their extreme rarity circulation consistent lack central for maintenance sensitivity.