Distribution of digestive proteinases in the alimentary tract of the european corn borer Ostrinia nubilalis (lepidoptera: Pyralidae)

摘要: The distribution of digestive proteinases in either the anterior and posterior midgut or between epithelium ectoperitrophic endo-peritrophic spaces were examined European corn borer, Ostrinia nubilalis. Trypsin, chymotrypsin, elastase, aminopeptidase activities same halves midgut. Of total activity, 95% was located epithelium, 90% trypsin, 97% 93% elastase activity found lumen. measured by hydrolysis benzoyl-L-arginine ethyl ester, chymotrypsin levels significantly higher space compared to endoperitrophic space. Digestion is proposed be sequential with tryptic digestion occurring Ingested protein digested further action a second trypsin. Final occurs an intracellular aminopeptidase. © 1995 Wiley-Liss, Inc.