A pilot study investigating a novel particle-based growth factor delivery system for preimplantation embryo culture.

摘要: STUDY QUESTION Can vascular endothelial growth factor (VEGF)-loaded silica supraparticles (V-SPs) be used as a novel mode of delivering VEGF to the developing preimplantation embryo in vitro? SUMMARY ANSWER Supplementation culture media with V-SPs promoted embryonic development manner equivalent supplemented free VEGF. WHAT IS KNOWN ALREADY is maternally derived that promotes vitro. However, its use clinical has limitations due low stability solution. DESIGN, SIZE, DURATION This study was laboratory-based analysis utilising mouse model. were prepared vitro and media. The bioactivity determined by blastocyst developmental outcomes (blastocyst rate total cell number). PARTICIPANTS/MATERIALS, SETTING, METHODS SPs loaded fluorescently labelled release kinetics characterised. Bioactivity unlabelled released from number) following individual 20 µl G1/G2 at 5% oxygen, 10 ng/ml recombinant solution or V-SPs. freeze-dried also assessed determine efficacy cryostorage. MAIN RESULTS AND THE ROLE OF CHANCE characterised an initial burst spheres followed consistent lower level over 48 h. resulted significant increases number relative control (P < 0.001), replicating effects medium freely fresh (P < 0.001). Similarly, freeze dried exerted comparable on (P < 0.05). LARGE SCALE DATA N/A. LIMITATIONS, REASONS FOR CAUTION In this proof principle study, only analysed WIDER IMPLICATIONS FINDINGS These findings suggest represent method which targeted dose therapeutic agents (e.g. bioactive VEGF) can delivered promote development, approach negates breakdown associated storage As such, may alternative effective embryo; however, potential IVF requires further investigation. FUNDING/COMPETING INTEREST(S) work funded University Melbourne. authors have no conflict interest declare.