Optical recording of trisynaptic pathway in rat hippocampal slices with a voltage-sensitive dye

摘要: Changes in membrane potentials were recorded from rat hippocampal slices with a voltage-sensitive dye using real-time optical recording system, which had high spatial resolution of 128 x points time 0.6 ms. Serial excitatory propagation was the dentate gyrus. CA3 and CA1 after stimulation perforant pathway, signals clearly divided into two components gyrus adjacent to stimulus site. The slow component suppressed Ca(2+)-free solution, but fast molecular layer not affected. However, application 1 microM tetrodotoxin fully abolished both components. These results suggest that mainly reflect Na(+)-dependent action postsynaptic potentials, respectively. response duration stratum radiatum significantly longer than other areas. long-lasting excitation is probably related associational projections, because direct pyramidal cell also produced similar results. dendritic important integrate synaptic transmission may be epileptogenesis. When long-term potentiation induced by tetanic (100 Hz for s), onset latency reduced as much 65%, suggesting an increase propagation. analysis spatial-temporal contributes understanding information processes hippocampus, learning memory including potentiation.