A comparison of centrifugation and tangential flow filtration for nanoparticle purification: A case study on acetalated dextran nanoparticles

摘要: Abstract Tangential flow filtration (TFF) is a purification method commonly used in multitude of fields, including particle engineering. Currently, there still lack comprehensive understanding the effects key TFF parameters on nanoparticle (NP) characteristics (purification outcomes). The present study aimed to investigate influence various factors TFF-purified NP. most NP method, centrifugation, was studied as control. A design experiment approach implemented transmembrane pressure, rate, and initial drug loading using paclitaxel model small molecule. Following determination optimized parameters, two methods were assessed other molecules (i.e. tacrolimus resveratrol). results indicated that played important roles purified outcomes. Compared with centrifugation resulted smaller higher loading. able circumvent issues associated conventional while yielding similar results, can potentially serve suitable large-scale for polymeric