Cholera toxin feeding did not induce oral tolerance in mice and abrogated oral tolerance to an unrelated protein antigen

摘要: The feeding of protein antigens to mice results in a state tolerance when is followed by parenteral immunization. Cholera toxin (CT) that has been used extensively as potent oral immunogen for mucosal IgA responses, but CT also stimulates substantial plasma IgG antibody response. This latter finding prompted us study whether or not induces tolerance. Mice were fed 5 mg keyhole limpet hemocyanin (KLH) 10 micrograms at least twice before immunization with 1 microgram KLH alum i.p. Plasma and intestinal secretions collected intervals. specific the samples was measured ELISA. Although did induce tolerance, any three mouse strains tested dose given orally. B subunit result either. When both together, able abrogate KLH, an antigenically unrelated protein. Moreover, along stimulated secretory anti-KLH whereas no such responses found alone. Thus, these experiments antigens, reciprocally linked occur simultaneously. appears stimulate altering regulatory environment gut-associated lymphoid tissue, shifting it toward responsiveness.