Isolation of a Leucoanthocyanidin Dioxygenase (LDOX) Gene from a Spray-type Chrysanthemum (Dendranthema × grandiflorum) and Its Colored Mutants
作者： Geung-Joo Lee , Si-Yong Kang , Sung Jin Chung , Hye Jung Lee , Dong Sub Kim
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摘要: A full-length cDNA and genomic DNA of a leucoanthocyanidin dioxygenase (DgLDOX) gene was isolated from the petals chrysanthemum ‘Argus’, comparative features among three flower color mutants derived gamma-ray mutagenesis were characterized. The coding region 1068 bp translated into 356 amino acids accordingly. size 1346 for while revealed ranges 1363 to 1374 bp. single intron between two exons DgLDOX found, which 112 but 128 or 137 mutants, indicating that insertion in occurred during mutagenesis. blot analysis presenting as copy genome. expressed both ‘Argus’ light-pink purple (AM1 AM3) had very weak expression only white mutant (AM2). results demonstrated variations might be associated with changes acid moieties fragment insertions gene, potentially resulted less colored mutant.
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cabdirect.org参考文章(42)
A. Menssen, S. Höhmann, W. Martin, P. S. Schnable, P. A. Peterson, H. Saedler, A. Gierl, The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. The EMBO Journal. ,vol. 9, pp. 3051- 3057 ,(1990) , 10.1002/J.1460-2075.1990.TB07501.X
T. Gerats, C. Martin, The control of flower coloration The molecular biology of flowering.. pp. 219- 255 ,(1993)
A. M. van Harten, Mutation Breeding: Theory and Practical Applications ,(1998)
Sunyoung Park, Younghee Kim, Identification and Characterization of Flavanone 3-Hydroxylase (F3H) Gene from Dendranthema grandiflora Horticulture Environment and Biotechnology. ,vol. 43, pp. 666- 670 ,(2002)
Jinwook Seo, Suk Weon Kim, Jonghyun Kim, Hyun Wook Cha, Jang R. Liu, Co-expression of flavonoid 3′ 5′-hydroxylase and flavonoid 3′-hydroxylase Accelerates Decolorization in Transgenic Chrysanthemum Petals Journal of Plant Biology. ,vol. 50, pp. 626- 631 ,(2007) , 10.1007/BF03030605
Atsushi Hoshino, Yasuyo Johzuka-Hisatomi, Shigeru Iida, Gene duplication and mobile genetic elements in the morning glories. Gene. ,vol. 265, pp. 1- 10 ,(2001) , 10.1016/S0378-1119(01)00357-2
Atsushi Tanaka, Ayako Sakamoto, Yasuhito Ishigaki, Osamu Nikaido, Guakin Sun, Yoshihiro Hase, Naoya Shikazono, Shigemitsu Tano, Hiroshi Watanabe, An Ultraviolet-B-Resistant Mutant with Enhanced DNA Repair in Arabidopsis Plant Physiology. ,vol. 129, pp. 64- 71 ,(2002) , 10.1104/PP.010894
Joseph Mol, Erich Grotewold, Ronald Koes, How genes paint flowers and seeds Trends in Plant Science. ,vol. 3, pp. 212- 217 ,(1998) , 10.1016/S1360-1385(98)01242-4
K. M. Davies, A Malus cDNA with homology to the Antirrhinum Candica and Zea A2 genes. Plant Physiology. ,vol. 103, pp. 1015- 1015 ,(1993) , 10.1104/PP.103.3.1015
Takashi Nakatsuka, Masahiro Nishihara, Keiichiro Mishiba, Saburo Yamamura, Two different mutations are involved in the formation of white-flowered gentian plants Plant Science. ,vol. 169, pp. 949- 958 ,(2005) , 10.1016/J.PLANTSCI.2005.06.013