Targeted Metabolomics Identifies Reliable and Stable Metabolites in Human Serum and Plasma Samples

摘要: Background Information regarding the variability of metabolite levels over time in an individual is required to estimate reproducibility measurements. In intervention studies, it critical appropriately judge changes that are elicited by any kind intervention. The pre-analytic phase (collection, transport and sample processing) a particularly important component data quality multi-center studies. Methods Reliability metabolites (within-and between-person variance, intraclass correlation coefficient) stability (shipment simulation at different temperatures, use gel-barrier collection tubes, freeze-thaw cycles) were analyzed fasting serum plasma samples 22 healthy human subjects using targeted LC-MS approach. Results measurements was higher compared good most saturated short-and medium-chain acylcarnitines, amino acids, biogenic amines, glycerophospholipids, sphingolipids hexose. majority stable for 24 h on cool packs room temperature non-centrifuged tubes. Plasma showed coherence. Serum concentrations mostly unaffected tube type one or two cycles. Conclusion A single point measurement assumed be sufficient metabolomics analysis metabolites. For shipment, should ideally separated frozen immediately after collection, as some acids amines become unstable within 3 packs. tubes can used safely this process they have no effect concentration Shipment cost-efficient alternative