Targeting of vascular adhesion protein-1 by positron emission tomography visualizes sites of inflammation in Borrelia burgdorferi-infected mice.

摘要: In the present study, we sought to evaluate feasibility of targeting vascular adhesion protein-1 (VAP-1) by positron emission tomography (PET) for longitudinal quantitative assessment Borrelia burgdorferi infection-induced inflammation in mice. Mice with B. arthritis were studied. During a 7-week follow-up period, progression was monitored weekly 68Ga-DOTA-Siglec-9 PET/computed (CT) and measurement tibiotarsal joint swellings. A subgroup infected mice treated ceftriaxone. Finally, histopathological performed VAP-1 expression joints determined. Explicit swelling uptake could be demonstrated affected from burgdorferi-infected By contrast, no obvious accumulation detected uninfected The maximum highest observed 4 weeks after infection. correlated (P < 0.0001) scoring (P = 0.020). Despite short-term antibiotic treatment, persisted, PET signal remained as high nontreated Immunohistochemistry revealed strong-to-moderate synovium mice, while only weak study showed that can detect Furthermore, PET/CT imaging allowed monitoring development over time.