A versatile split-beam ultracentrifuge recording scanner for use with multicell rotor and absorption optics.

摘要: A split-beam photoelectric scanner has been developed for use with multicell rotors and absorption optics. In essence, it was constructed by incorporating an electronic system sensing the orientation of a rotor (multiplexing system) into single cells. The single-cell used combination those Lamers et al. Spinco, several modifications. multiplexing designed according to following principle: time perform one revolution divided six equal intervals, using radius marker on counterbalance as reference point. Thus, successive intervals have one-to-one correspondence sextants rotor. position cell is recognized interval in which electric pulses are generated light bursts passing through cell. recognition effected set channels correlated sextants. Various performance tests made single- double-sector cells 2-cell, 4-cell, 6-cell at various speeds. Tests involving studies from spinning yielded results: (a) correspondence; (b) between two neighboring agree quantitatively their corresponding values, calculated angular separations sectors rotors; (c) relationships channel speeds observed were expected spatial rotor. In second series tests, ultracentrifuge patterns recorded studied. gave difference patterns, automatic correction nonuniform illumination, solution its solvent when contained either compartments or single-sector recorder response not influenced type, proportional absorbance sample. smooth Gaussian curves homogeneous deoxyribonucleic acids (DNA) nearly density banded simultaneously density-gradient columns. DNA curves, referred other density, agreed reported value. These results indicate that can be satisfactorily 6-cell, 2-cell rotors,