The Biosynthesis of C14- and H3-Labeled Insulin

摘要: In recent years, there has been much interest in the biosynthesis of insulin because its role diabetes. addition, exact amino acid sequence two peptide chains is known [1] and, therefore, molecule become a model protein for research synthetic mechanisms. Many techniques purification and characterization have developed [2,3,4]. study biosynthesis, several investigators incubated mammalian pancreas slices presence radioactive acids vitro, then isolated labeled [3,5,6] This approach limited beta cells islets Langerhans (which manufacture store insulin) comprise only small fraction total pancreas. During development certain teleost fish, however, islet tissue becomes separated from exocrine it appears adult as macroscopic aggregate endocrine called principal or Brockmann body. The contains which are functionally anatomically analogous to B-cells islets; fish similar structure biological activity [7,8]. Our studies on using were begun 1959; this report summary these investigations.