Simultaneous analysis of Alternaria toxins and citrinin in tomato: an optimised method using liquid chromatography-tandem mass spectrometry

摘要: Alternaria toxins and citrinin are mycotoxins produced by fungi growing on different raw materials agricultural commodities. Maximum levels of these in foods currently under consideration the European Commission as a risk management measure. In this study, new quantitative method is described for determination five tomato juice samples based LC-MS/MS detection. Samples were extracted with pure methanol, followed derivatisation step 2,4-dinitrophenylhydrazine to improve tenuazonic acid decrease wide polarity difference between compounds interest. purified hydrophilic-modified styrene polymer solid-phase extraction cartridges. High-performance liquid chromatographic columns packed core–shell tested separation C-18 phase was final applied achieve sufficient all relevant analytes. A key element approach prove successful transferability three triple quadrupole mass spectrometers. full single laboratory validation performed two systems performance characteristics met predefined requirements. Moreover, used an international proficiency test satisfactory z-scores obtained (−0.1 0.8 samples) demonstrated reliability described. The will be validated inter-laboratory collaborative study if criteria precision met, proposed Work Item Committee Standardisation.