Differing effects of dexamethasone and diclofenac on posterior capsule opacification-like changes in a rat lens explant model

摘要: Posterior capsular opacification (PCO) arises from lens cells that remain associated with the capsule after cataract surgery and subsequently become abnormal, proliferate migrate into visual pathway. In this study, a rat explant model was used to assess effects of prototype steroidal non-steroidal anti-inflammatory drugs, dexamethasone (DEX) diclofenac (DIC), on epithelial undergoing PCO-like changes. Such drugs are widely at time surgery. TGFbeta2 FGF-2 were added sequentially explants cultured for up 30 days, or without addition DEX DIC clinically relevant concentration. Without DIC, became multilayered tended retract plaques. Inclusion DEX, but not resulted in transient formation needle-like cells, enhanced cell coverage, retention monolayer migratory surrounding With drug addition, most aberrant, as indicated by loss Pax6 expression presence PCO markers alpha-smooth muscle actin type I collagen; however, both strongly collagen accumulation. Furthermore, coverage treated TGFbeta alone. Thus behaviour significantly differentially affected highlighting possibility control inflammation surgery, clinician's choice may influence development.