Gene amplification and drug resistance in cultured murine cells

摘要: Resistance of mouse cells to the folate analog, methotrexate, results from selection increasingly resistant on progressive increases methotrexate in culture medium. High-level resistance is associated with high rates synthesis dihydrofolate reductase and correspondingly numbers genes. In some variants gene copy number are stable absence pressure, whereas others they unstable. Analogies made antibiotic insecticide wherein organisms increased capacity counteract drug effect emergence resistance. Gene amplification may underlie many such phenomena.