Preparation of porous bioactive ceramic microspheres and in vitro osteoblastic culturing for tissue engineering application.

摘要: Abstract Microparticulates are useful for directly filling defective tissues as well delivering cells and bioactive molecules in regenerative medicine. This paper reports on the production of ceramic microspheres with an interconnected macropore structure. The sol–gel derived calcium silicate powder was homogenized oligomeric Camphene melt, which used a novel porogen, spherical-shaped microparticulates were obtained by oil-in-water emulsion method. A porous structure generated through sublimation within silicate–Camphene solidified blend under ambient conditions. retained crystalline phase apatite wollastonite during heat treatment induced phosphate precipitation body-simulating medium, showing characteristics bone-bioactive materials. Osteoblastic observed to anchor spread over surface microspheres, further proliferate actively culturing time. developed considered potentially regeneration hard matrix tissue engineering direct material.