Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
作者： Joanna Lapczuk-Romanska , Diana Busch , Ewa Gieruszczak , Agnieszka Drozdzik , Katarzyna Piotrowska
DOI: 10.3390/IJMS20194825
关键词:
摘要: Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance drug transporters measured using reliable quantification methods. Therefore, mRNA expression absolute content clinically relevant ABC (n = 6) SLC 15) family member in the human parotid gland, qRT-PCR liquid chromatography‒tandem mass spectrometry (LC−MS/MS) method, were studied. The nearly all proteins ranged between 0.04 0.45 pmol/mg (OCT3 > MRP1 PEPT2 MRP4 MATE1 BCRP). mRNAs ABCB1, ABCC2, ABCC3, SLC10A1, SLC10A2, SLC22A1, SLC22A5, SLC22A6, SLC22A7, SLC22A8, SLCO1A2, SLCO1B1, SLCO1B3 SLCO2B1 not detected. present study provides, for first time, membrane which could further be used to define salivary bidirectional transport (absorption secretion) mechanisms endogenous compounds xenobiotics.
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