Surface Immobilized His-tagged Azurin as a Model Interface for the Investigation of Vectorial Electron Transfer in Biological Systems
作者： Stefano Casalini , Marcello Berto , Alessandro Kovtun , Alessandra Operamolla , Giulia Di Rocco
DOI: 10.1016/J.ELECTACTA.2015.07.156
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摘要: Abstract A model system for the electrochemical investigation of vectorial electron transfer in biological systems was designed, assembled and characterized. Gold electrodes, functionalized with a -OCH 3 terminated, aromatic self-assembled monolayer, were used as substrate adsorption variants copper-containing, redox metalloprotein azurin. The engineered azurin bears polyhistidine tag at its C-terminus. Thanks to presence solvent exposed tag, which chelates Cu 2+ ions solution, we introduced an exogenous centre. different reduction potentials two centres their positioning respect surface are such that from copper centre electrode is mediated by native active site, closely paralleling processes naturally occurring multicentre metalloproteins.
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