Excision of deoxyribose phosphate residues by DNA polymerase beta during DNA repair
作者： Y Matsumoto , K Kim
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摘要: Eukaryotic DNA polymerase beta (pol beta) can catalyze synthesis during base excision repair. It is shown here that pol also catalyzes release of 5'-terminal deoxyribose phosphate (dRP) residues from incised apurinic-apyrimidinic sites, which are common intermediate products in The catalytic domain for this activity resides within an amino-terminal 8-kilodalton fragment beta, comprises a distinct structural the enzyme. Magnesium required dRP double-stranded but not single-stranded oligonucleotide. Analysis released indicates reaction occurs by beta-elimination rather than hydrolysis.
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