Improved in situ saccharification of cellulose pretreated by dimethyl sulfoxide/ionic liquid using cellulase from a newly isolated Paenibacillus sp. LLZ1.
作者： Liangzhi Li , Cuiying Hu , Lishi Yan , Tianyun Wu , Jiaolong Fu
DOI: 10.1016/J.BIORTECH.2015.11.039
关键词:
摘要: A cellulase producing strain was newly isolated from soil samples and identified as Paenibacillus sp. LLZ1. novel aqueous-dimethyl sulfoxide (DMSO)/1-ethyl-3-methylimidazolium diethyl phosphate ([Emin]DEP)-cellulase system designed optimized. In the pretreatment, DMSO found to be a low-cost substitute of up 70% ionic liquid enhance cellulose dissolution. enzymatic saccharification, optimum pH temperature LLZ1 were 6.0 40°C, respectively. Under optimized reaction condition, conversion microcrystalline bagasse increased by 39.3% 37.6%, compared with unpretreated cellulose. Compared current methods this new approach has several advantages including lower operating temperature, milder pH, less usage liquid, indicating marked progress in environmental friendly hydrolysis biomass-based materials.
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