Modulation of corneal fibroblast contractility within fibrillar collagen matrices.

摘要: PURPOSE. To investigate the migratory and contractile behavior of isolated human corneal fibroblasts in fibrillar collagen matrices. METHODS. A telomerase-infected, extended-lifespan fibroblast cell line (HTK) was transfected by using a vector for enhanced green fluorescent protein (GFP)--actinin. Cells were plated at low density on top or within 100-m-thick lattices. After 18 hours to 7 days, time-lapse imaging performed. At each 1- 3-minute interval, GFP Nomarski differential interference contrast (DIC) images acquired rapid succession. Serum-containing (S) medium used initially perfusion. 2 hours, perfusion switched either serum-free (S )o r S containing Rho-kinase inhibitor Y-27632 1 hours. Finally, changed back hour. RESULTS. Two 4 days after plating, many cells underwent spontaneous contraction and/or relaxation medium. decrease distance between consecutive -actinin‐ dense bodies along stress fibers measured during contraction, focal adhesion matrix displacements correlated significantly. Removal serum inhibition induced body elongation stress, as confirmed finite element modeling. Rapid formation extension pseudopodia filopodia also observed, transient tractional forces generated these extending processes. CONCLUSIONS. Cultured can undergo changes subcellular pattern force generation that are mediated, part, Rho-kinase. Sarcomeric shortening contracting is demonstrated first time. (Invest Ophthalmol Vis Sci. 2003;44:4724 ‐ 4735) DOI:10.1167/iovs.03-0513