Methods and compositions for universal size-specific PCR
作者： Min Seob Lee , Yanfeng Yang
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摘要: Provided herein are products and processes for the amplification, detection sequencing of short-stranded nucleic acid in presence a high background long-stranded genomic material (e.g., host or maternal acids). The methods rely on use inside outside primers introduced at varying concentrations, as well universal amplification reactions that preferentially amplify short, low copy number acid.
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