Development of a multiplex amplification refractory mutation system for simultaneous authentication of Korean ginseng cultivars “Gumpoong” and “Chungsun”
作者： Hongtao Wang , Woo-Saeng Kwon , Dong-Uk Yang , Min-Kyeoung Kim , Subramaniyam Sathiyamoorthy
DOI: 10.3109/19401736.2011.588216
关键词:
摘要: BACKGROUND AND AIMS. Molecular authentication of Korean ginseng cultivars was investigated using the mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 7 (nad7) intron 3 region. MATERIALS METHODS. A mutation site specific to Panax "Gumpoong" and "Chungsun" detected within sequence data. Based on this "Gumpoong"-specific single nucleotide polymorphism reported in 26S rDNA, two modified allele-specific primer pairs were designed a multiplex amplification refractory system (MARMS) applied identify "Chungsun." RESULTS. The results showed that can be clearly discriminated from other by simultaneously identifying haplotype allele applying MARMS. CONCLUSION. This study, therefore, provides simple reliable method for simultaneous cultivars.
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