The Automated Determination of Serum Uric Acid

作者: Stanley Morgenstern , Richard V Flor , James H Kaufman , Bernard Klein

DOI: 10.1093/CLINCHEM/12.11.748

关键词:

摘要: An automated procedure is presented for the enzymatic determination of serum uric acid on both AutoAnalyzer and Robot Chemist. The measures as neocuproine complex, difference in amount Cu+ formed by reaction a Cu++-alkanolamine buffered solution with under precisely controlled conditions before after uricase treatment serum. proportional to true content. elements contributing reaction, colorimetric elimination interferences were investigated. Comparison values obtained this method those ultraviolet spectrophotometry show very good agreement.

参考文章(8)
Clyde A. Dubbs, Frances W. Davis, William S. Adams, Simple microdetermination of uric acid. Journal of Biological Chemistry. ,vol. 218, pp. 497- 504 ,(1956) , 10.1016/S0021-9258(18)65912-5
Oliver H. Buchanan, Walter D. Block, A.A. Christman, THE METABOLISM OF THE METHYLATED PURINES: I. THE ENZYMATIC DETERMINATION OF URINARY URIC ACID Journal of Biological Chemistry. ,vol. 157, pp. 181- 187 ,(1945) , 10.1016/S0021-9258(17)41641-3
Harold A. Bulger, Helen E. Johns, THE DETERMINATION OF PLASMA URIC ACID Journal of Biological Chemistry. ,vol. 140, pp. 427- 440 ,(1941) , 10.1016/S0021-9258(18)51332-6
Naomi C. Geib, Walter D. Block, An enzymatic method for the determination of uric acid in whole blood. Journal of Biological Chemistry. ,vol. 168, pp. 747- 756 ,(1947) , 10.1016/S0021-9258(17)30933-X
E. Prætorius, An Enzymatic Method for the Determination of Uric Acid by Ultraviolet Spectrofhotometry Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 1, pp. 222- 230 ,(1949) , 10.3109/00365514909069945
Leonard V Crowley, DETERMINATION OF URIC ACID: AN AUTOMATED ANALYSIS BASED ON A CARBONATE METHOD. Clinical Chemistry. ,vol. 10, pp. 838- 844 ,(1964) , 10.1093/CLINCHEM/10.9.838