Distribution of glucuronic and iduronic acid units in heparin chains.
作者： S Radoff , I Danishefsky
DOI: 10.1016/S0021-9258(18)95708-X
关键词:
摘要: Abstract The distribution of glucuronic and iduronic acid within the chains anticoagulantly active inactive beef lung heparin was investigated. A fraction with an average molecular weight 19,500 isolated from heterodisperse mixture then separated into components by affinity chromatography. Each sample linked through its reducing terminus to tyramine, reduced sodium borotritide, bound covalently Sepharose via azo bridge. treated a limited amount HNO2 degraded fragments were removed. sections chain contiguous original detached insoluble matrix reaction dithionite. recovered polysaccharide fractionated according size on Sephadex G-200 each uronic in individual fractions determined. Inactive showed constant percentage all fragments, i.e. about 8.9% total acid. With increased distance chain, ranging 9.5 20% acids. These results suggest that biosynthesis involves unique reactions or specific processing macromolecule.
sci-hub.st 参考文章(18)
Noboru OTOTANI, Masaki KIKUCHI, Zensaku YOSIZAWA, Comparative Studies on the Structures of Highly Active and Relatively Inactive Forms of Whale Heparin Journal of Biochemistry. ,vol. 90, pp. 241- 246 ,(1981) , 10.1093/OXFORDJOURNALS.JBCHEM.A133456
U Lindahl, L Thunberg, G Bäckström, J Riesenfeld, K Nordling, I Björk, Extension and structural variability of the antithrombin-binding sequence in heparin. Journal of Biological Chemistry. ,vol. 259, pp. 12368- 12376 ,(1984) , 10.1016/S0021-9258(18)90755-6
HC Robinson, AA Horner, M Höök, S Ogren, U Lindahl, None, A proteoglycan form of heparin and its degradation to single-chain molecules. Journal of Biological Chemistry. ,vol. 253, pp. 6687- 6693 ,(1978) , 10.1016/S0021-9258(17)37973-5
Norman F. Boas, Method for the determination of hexosamines in tissues. Journal of Biological Chemistry. ,vol. 204, pp. 553- 563 ,(1953) , 10.1016/S0021-9258(18)66055-7
Sadako Inoue, The determination of iduronic acid and glucuronic acid in heparins by a new technique: Re-evaluation of the variable hexuronic acid composition of mammalian heparins Biochimica et Biophysica Acta. ,vol. 329, pp. 264- 270 ,(1973) , 10.1016/0304-4165(73)90291-2
D.D. Metcalfe, J.A. Smith, K.F. Austen, J.E. Silbert, Polydispersity of rat mast cell heparin. Implications for proteoglycan assembly. Journal of Biological Chemistry. ,vol. 255, pp. 11753- 11758 ,(1980) , 10.1016/S0021-9258(19)70198-7
A A Horner, E Young, Asymmetric distribution of sites with high affinity for antithrombin III in rat skin heparin proteoglycans. Journal of Biological Chemistry. ,vol. 257, pp. 8749- 8754 ,(1982) , 10.1016/S0021-9258(18)34192-9
S Radoff, I Danishefsky, Location on heparin of the oligosaccharide section essential for anticoagulant activity. Journal of Biological Chemistry. ,vol. 259, pp. 166- 172 ,(1984) , 10.1016/S0021-9258(17)43636-2
Steven Radoff, Isidore Danishefsky, Solid-phase synthesis of fluorescent heparin Analytical Biochemistry. ,vol. 120, pp. 373- 378 ,(1982) , 10.1016/0003-2697(82)90360-8
R. L. Taylor, J. E. Shively, H. E. Conrad, J. A. Cifonelli, Uronic acid composition of heparins and heparan sulfates Biochemistry. ,vol. 12, pp. 3633- 3637 ,(1973) , 10.1021/BI00743A010