Effects of ethanol ingestion and dietary fat levels on mitochondrial lipids in male and female rats.

摘要: The effects of sex, dietary fat levels, and ethanol ingestion on rat liver mitochondrial lipids have been studied. Two groups male animals were fed either a low-fat diet for about 76 days or high-fat 52 days, two female the same 50 37 days. Ethanol was substituted isocalorically carbohydrate amounted to 36% total calories. as well individual concentrations fatty acids, phospholipids, neutral determined in all eight animals. Variable changes observed acid composition mitochondria from each four After ingestion, there decrease arachidonate/linoleate ratio males, while no change females. Increasing content decreased this both controls experimentals, but it did not alter sex. Presumably, due fact that corn oil only source lipid. concentration increased except males diet. A group. pattern reflected phospholipid concentrations. In case, majority could be accounted by phosphatidylcholine (PC). Measurement choline oxidase (C.O.) showed C.O. activity group males. No other three groups. Chronic is known increase methylation phosphatidylethanolamine (PE); therefore, order PC, must sufficient magnitude offset PE methylation. effect Neither nor exerted much These results emphasize importance levels sex study structure function relation metabolism.