Identification, quantification, and functional aspects of skeletal muscle protein-carbonylation in vivo during acute oxidative stress.

摘要: Reactive oxidative species (ROS) play important roles in cellular signaling but can also modify and often functionally inactivate other biomolecules. Thus, cells have developed effective enzymatic nonenzymatic strategies to scavenge ROS. However, under stress, ROS production is able overwhelm the scavenging systems, increasing levels of impaired proteins. A major class irreversible modifications carbonylation, which refers reactive carbonyl-groups. In this investigation, we studied clearance rates for skeletal muscle proteins a rat model acute stress over time period 24 h using gel-based proteomics approach. Optimized ELISA Western blots with 10-fold improved sensitivities showed that carbonylation level was stable at 4 nmol per mg protein 3 following induction. The then increased 3-fold 6 remained stable. total, changed steady state 20 resulted 38 Carbonylation these followed diverse kinetics some being highly carbonylated very quickly, whereas others peaked 9 sample or continued increase up after induced.