Anti-inflammatory and antioxidant effects of cerium oxide nanoparticles in human endothelial cells

作者: S. Del Turco , G. Ciofani , V. Cappello , T. Navarra , C. Caselli

DOI: 10.1093/EURHEARTJ/EHT309.P4174

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摘要: Purpose: The vascular endothelium, by virtue of its strategic location and important role in cardiovascular disease, represents an target for drug or gene therapy. Studies have focused on targeting the endothelium with nanoparticles, as therapeutic agents. Cerium oxide nanoparticles (nanoceria) attracted much attention due to their wide range beneficial effects. We evaluated cytocompatibility anti-inflammatory anti-oxidant effects nanoceria endothelial umbilical vein endothelail cells (HUVECs). Methods results: HUVECs were incubated 24 48h cell culture medium at 50 μg/mL. Cell viability was assayed WST-1 assay, adhesion molecule (VCAM-1) expression surface enzyme immunoassay, intracellular ROS production fluorescent probe 6-carboxy-2'-7'-dichlorofluoresceine, cytokine ELISA assay interaction nanoparticles/cells transmission electron microscopy (TEM). Nanoceria did not affect growth vitality any incubation time. reduced H2O2 (25 μmol/L)-induced a time-dependent manner (-40%±10%, -26%±8%, p<0.05, 48 h, respectively) VCAM-1 exposure induced TNF-α ng/mL). (-35%± 6% p<0.01). Moreover, significantly IL-6 (38±1.9 pg/mL 34±1.5 vs 45±2.25 pg/mL, respectively, p<0.05) Il-8 (633±31 807±40 885±27 release after stimulation TNF-α. TEM analysis revealed that uptake 24h localization found mostly cytoplasm without preferential subcellular site. Conclusions: These findings highlight antioxidant antiinflammatory activity combined lack toxicity, makes it extremely promising tool.

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