Flexible amine-functionalized triphenylamine derivative as a fluorescent “light-up” probe for G-quadruplex DNA
作者： Ming-Qi Wang , Shuo Liu , Cai-Ping Tang , Aun Raza , Shuo Li
DOI: 10.1016/J.DYEPIG.2016.08.041
关键词:
摘要: Abstract In this study, a flexible amine-functionalized triphenylamine dye 2 was developed and utilized as G4 DNA fluorescent probe. The selectivity, sensitivity binding properties of the interacting with DNAs were investigated. illustrated in fluorescence titration assays preferentially bound significant “light-up” effect. detection limit value for below 10 nM solution. CD spectroscopy analysis revealed that could promote G-rich sequence to adapt an antiparallel structure. These results indicated showed stronger affinity response than our previous reported triphenylamine-quinolinium scaffold ( 1b ) due incorporation amine moiety. Therefore, study gave certain crucial factors on developing effective probes DNA. cellular application cancer cells HepG2 also demonstrated.
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