Suppressive effect of tetraprenylacetone on gastric atrophy induced by short-term administration of N-methyl-N′-nitro- N-nitrosoguanidine in rats

摘要: Background: Several studies have been reported on the effects of various therapeutic agents in enhancing or suppressing carcinogenic activity N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). However, it is still unknown whether a mucosal protective agent could suppress its activity. Methods: Twenty-five Wistar male rats were divided into four groups: group 1, MNNG alone; 2, MNNG + tetraprenylacetone; 3, control; 4, tetraprenylacetone alone. 100 mg/mL was freely given to groups 1 and (200 mg/kg intraperitoneal) additionally administered every other day 2 4. The animals sacrificed at 10 weeks gastric mucosa examined. Results: Atrophic changes observed antrum after 8 oral administration MNNG. Furthermore, using immunohistological analysis with 5-bromo-2′-deoxyuridine (BrdU), proliferative zone found be enlarged shifted upward, although BrdU labelling index unaltered. Intraperitoneal suppressed MNNG-induced atrophic change alterations markers. Tetraprenylacetone alone did not an effect either morphological markers. Conclusion: These observations suggest that defensive factors may play critical roles inducing carcinogenesis by exogenic carcinogen.